Construct: shRNA TRCN0000041086

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011931.1-1427s1c1
DNA Barcode:
GCTGTATCAGTTGGAGTAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rfwd2 (26374)
Gene Description:
ring finger and WD repeat domain 2
Transcript:
RefSeq NM_011931.1 (NON-CURRENT)
Match location:
Position 1427 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041086)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTATCAGTTGGAGTAGTT
Hairpin Sequence:
5'-CCGG-GCTGTATCAGTTGGAGTAGTT-CTCGAG-AACTACTCCAACTGATACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTATCAGTTGGAGTAGTTCTCGAGAACTACTCCAACTGATACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTATCAGTTGGAGTAGTTCTCGAGAACTACTCCAACTGATACAGC-3'

Other clones with same target sequence:

  1. TRCN0000363790