Construct: shRNA TRCN0000041144

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016694.2-867s1c1
DNA Barcode:
CGTGATCTGTTTGGACTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Park2 (50873)
Gene Description:
Parkinson disease (autosomal recessive, juvenile) 2, parkin
Transcript:
RefSeq NM_016694.2 (NON-CURRENT)
Match location:
Position 867 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041144)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGATCTGTTTGGACTGTTT
Hairpin Sequence:
5'-CCGG-CGTGATCTGTTTGGACTGTTT-CTCGAG-AAACAGTCCAAACAGATCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGATCTGTTTGGACTGTTTCTCGAGAAACAGTCCAAACAGATCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGATCTGTTTGGACTGTTTCTCGAGAAACAGTCCAAACAGATCACG-3'

Other clones with same target sequence:

(none)