Construct: shRNA TRCN0000041167

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023835.1-880s1c1
DNA Barcode:
GCAGAGTCTGAAAGTGAGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim12a (76681)
Gene Description:
tripartite motif-containing 12A
Transcript:
RefSeq NM_023835.1 (NON-CURRENT)
Match location:
Position 880 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041167)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGTCTGAAAGTGAGCAT
Hairpin Sequence:
5'-CCGG-GCAGAGTCTGAAAGTGAGCAT-CTCGAG-ATGCTCACTTTCAGACTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGTCTGAAAGTGAGCATCTCGAGATGCTCACTTTCAGACTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGTCTGAAAGTGAGCATCTCGAGATGCTCACTTTCAGACTCTGC-3'

Other clones with same target sequence:

(none)