Construct: shRNA TRCN0000041217

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172300.2-583s1c1
DNA Barcode:
CTCGGGTCAAACTGATGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ube2z (268470)
Gene Description:
ubiquitin-conjugating enzyme E2Z
Transcript:
RefSeq NM_172300.2 (NON-CURRENT)
Match location:
Position 583 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041217)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCGGGTCAAACTGATGACAA
Hairpin Sequence:
5'-CCGG-CTCGGGTCAAACTGATGACAA-CTCGAG-TTGTCATCAGTTTGACCCGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCGGGTCAAACTGATGACAACTCGAGTTGTCATCAGTTTGACCCGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCGGGTCAAACTGATGACAACTCGAGTTGTCATCAGTTTGACCCGAG-3'

Other clones with same target sequence:

(none)