Construct: shRNA TRCN0000041232

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_356116.1-295s1c1
DNA Barcode:
CATGTTTGTGATGGGTGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC382043 (382043)
Gene Description:
similar to Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Transcript:
RefSeq XM_356116.1 (NON-CURRENT)
Match location:
Position 295 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041232)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGTTTGTGATGGGTGTGAA
Hairpin Sequence:
5'-CCGG-CATGTTTGTGATGGGTGTGAA-CTCGAG-TTCACACCCATCACAAACATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGTTTGTGATGGGTGTGAACTCGAGTTCACACCCATCACAAACATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGTTTGTGATGGGTGTGAACTCGAGTTCACACCCATCACAAACATG-3'

Other clones with same target sequence:

(none)