Construct: shRNA TRCN0000041241

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_128212.2-308s1c1
DNA Barcode:
GCACTTCGTATAAAGACCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC223928 (223928)
Gene Description:
similar to Hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit
Transcript:
RefSeq XM_128212.2 (NON-CURRENT)
Match location:
Position 308 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041241)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTTCGTATAAAGACCTAA
Hairpin Sequence:
5'-CCGG-GCACTTCGTATAAAGACCTAA-CTCGAG-TTAGGTCTTTATACGAAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTTCGTATAAAGACCTAACTCGAGTTAGGTCTTTATACGAAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTTCGTATAAAGACCTAACTCGAGTTAGGTCTTTATACGAAGTGC-3'

Other clones with same target sequence:

(none)