Construct: shRNA TRCN0000041249

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009466.2-377s1c1
DNA Barcode:
CCTATTTATGAGCCTGGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ugdh (22235)
Gene Description:
UDP-glucose dehydrogenase
Transcript:
RefSeq NM_009466.2 (CURRENT)
Match location:
Position 377 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041249)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATTTATGAGCCTGGATTA
Hairpin Sequence:
5'-CCGG-CCTATTTATGAGCCTGGATTA-CTCGAG-TAATCCAGGCTCATAAATAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATTTATGAGCCTGGATTACTCGAGTAATCCAGGCTCATAAATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATTTATGAGCCTGGATTACTCGAGTAATCCAGGCTCATAAATAGG-3'

Other clones with same target sequence:

(none)