Construct: shRNA TRCN0000041266
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_139735.2-265s1c1
- DNA Barcode:
- GATGCCGATGCTGAATATGTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC224508 (224508)
- Gene Description:
- similar to Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (38 kDa BFA-dependent ADP-ribosylation substrate) (BARS-38)
- Transcript:
- RefSeq XM_139735.2 (NON-CURRENT)
- Match location:
- Position 265 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000041266)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GATGCCGATGCTGAATATGTT
- Hairpin Sequence:
-
5'-CCGG-GATGCCGATGCTGAATATGTT-CTCGAG-AACATATTCAGCATCGGCATC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGATGCCGATGCTGAATATGTTCTCGAGAACATATTCAGCATCGGCATCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGATGCCGATGCTGAATATGTTCTCGAGAACATATTCAGCATCGGCATC-3'
Other clones with same target sequence:
(none)
