Construct: shRNA TRCN0000041269

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007381.2-552s1c1
DNA Barcode:
GAAGTGACTTACAAGGAGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acadl (11363)
Gene Description:
acyl-Coenzyme A dehydrogenase, long-chain
Transcript:
RefSeq NM_007381.2 (NON-CURRENT)
Match location:
Position 552 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041269)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTGACTTACAAGGAGTAA
Hairpin Sequence:
5'-CCGG-GAAGTGACTTACAAGGAGTAA-CTCGAG-TTACTCCTTGTAAGTCACTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTGACTTACAAGGAGTAACTCGAGTTACTCCTTGTAAGTCACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTGACTTACAAGGAGTAACTCGAGTTACTCCTTGTAAGTCACTTC-3'

Other clones with same target sequence:

  1. TRCN0000324806