Construct: shRNA TRCN0000041323

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_123164.2-460s1c1
DNA Barcode:
GACATCAAGAAGGTGGTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC224923 (224923)
Gene Description:
similar to glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (EC 1.2.1.12)
Transcript:
RefSeq XM_123164.2 (NON-CURRENT)
Match location:
Position 460 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041323)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACATCAAGAAGGTGGTGAAA
Hairpin Sequence:
5'-CCGG-GACATCAAGAAGGTGGTGAAA-CTCGAG-TTTCACCACCTTCTTGATGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACATCAAGAAGGTGGTGAAACTCGAGTTTCACCACCTTCTTGATGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACATCAAGAAGGTGGTGAAACTCGAGTTTCACCACCTTCTTGATGTC-3'

Other clones with same target sequence:

(none)