Construct: shRNA TRCN0000041349

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178878.1-1465s1c1
DNA Barcode:
GCTCTCCCAATCAATCAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hadha (97212)
Gene Description:
hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Transcript:
RefSeq NM_178878.1 (NON-CURRENT)
Match location:
Position 1465 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041349)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCCCAATCAATCAAATT
Hairpin Sequence:
5'-CCGG-GCTCTCCCAATCAATCAAATT-CTCGAG-AATTTGATTGATTGGGAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCCCAATCAATCAAATTCTCGAGAATTTGATTGATTGGGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCCCAATCAATCAAATTCTCGAGAATTTGATTGATTGGGAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000315484