Construct: shRNA TRCN0000041371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007437.1-507s1c1
DNA Barcode:
GCCATTGTTAAGCCCTCAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aldh3a2 (11671)
Gene Description:
aldehyde dehydrogenase family 3, subfamily A2
Transcript:
RefSeq NM_007437.1 (NON-CURRENT)
Match location:
Position 507 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATTGTTAAGCCCTCAGAA
Hairpin Sequence:
5'-CCGG-GCCATTGTTAAGCCCTCAGAA-CTCGAG-TTCTGAGGGCTTAACAATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATTGTTAAGCCCTCAGAACTCGAGTTCTGAGGGCTTAACAATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATTGTTAAGCCCTCAGAACTCGAGTTCTGAGGGCTTAACAATGGC-3'

Other clones with same target sequence:

  1. TRCN0000308654