Construct: shRNA TRCN0000041456

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145424.1-726s1c1
DNA Barcode:
GTGCCAGATTATGCTCAAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rdh16f2 (216454)
Gene Description:
RDH16 family member 2
Transcript:
RefSeq NM_145424.1 (NON-CURRENT)
Match location:
Position 726 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041456)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCCAGATTATGCTCAAATA
Hairpin Sequence:
5'-CCGG-GTGCCAGATTATGCTCAAATA-CTCGAG-TATTTGAGCATAATCTGGCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCCAGATTATGCTCAAATACTCGAGTATTTGAGCATAATCTGGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCCAGATTATGCTCAAATACTCGAGTATTTGAGCATAATCTGGCAC-3'

Other clones with same target sequence:

(none)