Construct: shRNA TRCN0000041466

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053080.2-448s1c1
DNA Barcode:
CGACCTGGAAGGCTGTATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aldh1a3 (56847)
Gene Description:
aldehyde dehydrogenase family 1, subfamily A3
Transcript:
RefSeq NM_053080.2 (NON-CURRENT)
Match location:
Position 448 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041466)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACCTGGAAGGCTGTATTAA
Hairpin Sequence:
5'-CCGG-CGACCTGGAAGGCTGTATTAA-CTCGAG-TTAATACAGCCTTCCAGGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACCTGGAAGGCTGTATTAACTCGAGTTAATACAGCCTTCCAGGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACCTGGAAGGCTGTATTAACTCGAGTTAATACAGCCTTCCAGGTCG-3'

Other clones with same target sequence:

(none)