Construct: shRNA TRCN0000041492

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145567.1-136s1c1
DNA Barcode:
AGCGGTGTGTTCTAGGTCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hibadh (58875)
Gene Description:
3-hydroxyisobutyrate dehydrogenase
Transcript:
RefSeq NM_145567.1 (CURRENT)
Match location:
Position 136 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041492)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCGGTGTGTTCTAGGTCAAT
Hairpin Sequence:
5'-CCGG-AGCGGTGTGTTCTAGGTCAAT-CTCGAG-ATTGACCTAGAACACACCGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCGGTGTGTTCTAGGTCAATCTCGAGATTGACCTAGAACACACCGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCGGTGTGTTCTAGGTCAATCTCGAGATTGACCTAGAACACACCGCT-3'

Other clones with same target sequence:

  1. TRCN0000324580