Construct: shRNA TRCN0000041526

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016763.1-813s1c1
DNA Barcode:
GCTCATCTGGTACAGACCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hsd17b10 (15108)
Gene Description:
hydroxysteroid (17-beta) dehydrogenase 10
Transcript:
RefSeq NM_016763.1 (NON-CURRENT)
Match location:
Position 813 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041526)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCATCTGGTACAGACCATA
Hairpin Sequence:
5'-CCGG-GCTCATCTGGTACAGACCATA-CTCGAG-TATGGTCTGTACCAGATGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCATCTGGTACAGACCATACTCGAGTATGGTCTGTACCAGATGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCATCTGGTACAGACCATACTCGAGTATGGTCTGTACCAGATGAGC-3'

Other clones with same target sequence:

(none)