Construct: shRNA TRCN0000041631

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008292.2-1209s1c1
DNA Barcode:
GCTGTCATTCAACTTTGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hsd17b4 (15488)
Gene Description:
hydroxysteroid (17-beta) dehydrogenase 4
Transcript:
RefSeq NM_008292.2 (NON-CURRENT)
Match location:
Position 1209 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041631)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTCATTCAACTTTGCAAA
Hairpin Sequence:
5'-CCGG-GCTGTCATTCAACTTTGCAAA-CTCGAG-TTTGCAAAGTTGAATGACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTCATTCAACTTTGCAAACTCGAGTTTGCAAAGTTGAATGACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTCATTCAACTTTGCAAACTCGAGTTTGCAAAGTTGAATGACAGC-3'

Other clones with same target sequence:

(none)