Construct: shRNA TRCN0000041681

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008294.1-721s1c1
DNA Barcode:
GCACTGAAGAACAACAGCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hsd3b4 (15495)
Gene Description:
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 4
Transcript:
RefSeq NM_008294.1 (NON-CURRENT)
Match location:
Position 721 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041681)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTGAAGAACAACAGCATA
Hairpin Sequence:
5'-CCGG-GCACTGAAGAACAACAGCATA-CTCGAG-TATGCTGTTGTTCTTCAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTGAAGAACAACAGCATACTCGAGTATGCTGTTGTTCTTCAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTGAAGAACAACAGCATACTCGAGTATGCTGTTGTTCTTCAGTGC-3'

Other clones with same target sequence:

(none)