Construct: shRNA TRCN0000041754

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001978.1-835s1c1
DNA Barcode:
TGACATCAAGAAGGTGGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm5138 (380687)
Gene Description:
predicted gene 5138; glyceraldehyde-3-phosphate dehydrogenase pseudogene
Transcript:
RefSeq NM_001001978.1 (NON-CURRENT)
Match location:
Position 835 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041754)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACATCAAGAAGGTGGTGAA
Hairpin Sequence:
5'-CCGG-TGACATCAAGAAGGTGGTGAA-CTCGAG-TTCACCACCTTCTTGATGTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACATCAAGAAGGTGGTGAACTCGAGTTCACCACCTTCTTGATGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACATCAAGAAGGTGGTGAACTCGAGTTCACCACCTTCTTGATGTCA-3'

Other clones with same target sequence:

(none)