Construct: shRNA TRCN0000041818

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026614.1-378s1c1
DNA Barcode:
AGGTGATTCTTCAGGCTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndufa5 (68202)
Gene Description:
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5
Transcript:
RefSeq NM_026614.1 (NON-CURRENT)
Match location:
Position 378 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041818)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTGATTCTTCAGGCTGAAA
Hairpin Sequence:
5'-CCGG-AGGTGATTCTTCAGGCTGAAA-CTCGAG-TTTCAGCCTGAAGAATCACCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTGATTCTTCAGGCTGAAACTCGAGTTTCAGCCTGAAGAATCACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTGATTCTTCAGGCTGAAACTCGAGTTTCAGCCTGAAGAATCACCT-3'

Other clones with same target sequence:

(none)