Construct: shRNA TRCN0000041845

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010887.1-373s1c1
DNA Barcode:
AGTGCCAAAGAAGATGCAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndufs4 (17993)
Gene Description:
NADH dehydrogenase (ubiquinone) Fe-S protein 4
Transcript:
RefSeq NM_010887.1 (NON-CURRENT)
Match location:
Position 373 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041845)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGCCAAAGAAGATGCAATT
Hairpin Sequence:
5'-CCGG-AGTGCCAAAGAAGATGCAATT-CTCGAG-AATTGCATCTTCTTTGGCACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGCCAAAGAAGATGCAATTCTCGAGAATTGCATCTTCTTTGGCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGCCAAAGAAGATGCAATTCTCGAGAATTGCATCTTCTTTGGCACT-3'

Other clones with same target sequence:

  1. TRCN0000312044