Construct: shRNA TRCN0000041855

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133666.1-1178s1c1
DNA Barcode:
CGCTCGACAGACATTGTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndufv1 (17995)
Gene Description:
NADH dehydrogenase (ubiquinone) flavoprotein 1
Transcript:
RefSeq NM_133666.1 (NON-CURRENT)
Match location:
Position 1178 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041855)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTCGACAGACATTGTGAAA
Hairpin Sequence:
5'-CCGG-CGCTCGACAGACATTGTGAAA-CTCGAG-TTTCACAATGTCTGTCGAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTCGACAGACATTGTGAAACTCGAGTTTCACAATGTCTGTCGAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTCGACAGACATTGTGAAACTCGAGTTTCACAATGTCTGTCGAGCG-3'

Other clones with same target sequence:

  1. TRCN0000309460