Construct: shRNA TRCN0000041885

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008710.2-1240s1c1
DNA Barcode:
CGGGAGAACTTTACGTTCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nnt (18115)
Gene Description:
nicotinamide nucleotide transhydrogenase
Transcript:
RefSeq NM_008710.2 (NON-CURRENT)
Match location:
Position 1240 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041885)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAGAACTTTACGTTCATA
Hairpin Sequence:
5'-CCGG-CGGGAGAACTTTACGTTCATA-CTCGAG-TATGAACGTAAAGTTCTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAGAACTTTACGTTCATACTCGAGTATGAACGTAAAGTTCTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAGAACTTTACGTTCATACTCGAGTATGAACGTAAAGTTCTCCCG-3'

Other clones with same target sequence:

  1. TRCN0000309453