Construct: shRNA TRCN0000041894

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010941.3-585s1c1
DNA Barcode:
GCCGGAGTTCAGAAACTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nsdhl (18194)
Gene Description:
NAD(P) dependent steroid dehydrogenase-like
Transcript:
RefSeq NM_010941.3 (CURRENT)
Match location:
Position 585 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041894)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGGAGTTCAGAAACTCATT
Hairpin Sequence:
5'-CCGG-GCCGGAGTTCAGAAACTCATT-CTCGAG-AATGAGTTTCTGAACTCCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGGAGTTCAGAAACTCATTCTCGAGAATGAGTTTCTGAACTCCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGGAGTTCAGAAACTCATTCTCGAGAATGAGTTTCTGAACTCCGGC-3'

Other clones with same target sequence:

  1. TRCN0000312096