Construct: shRNA TRCN0000041895

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010941.3-360s1c1
DNA Barcode:
CGAGGCTATACTGTCAATGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nsdhl (18194)
Gene Description:
NAD(P) dependent steroid dehydrogenase-like
Transcript:
RefSeq NM_010941.3 (CURRENT)
Match location:
Position 360 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041895)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGCTATACTGTCAATGTA
Hairpin Sequence:
5'-CCGG-CGAGGCTATACTGTCAATGTA-CTCGAG-TACATTGACAGTATAGCCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGCTATACTGTCAATGTACTCGAGTACATTGACAGTATAGCCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGCTATACTGTCAATGTACTCGAGTACATTGACAGTATAGCCTCG-3'

Other clones with same target sequence:

(none)