Construct: shRNA TRCN0000041896
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_010941.3-1142s1c1
- DNA Barcode:
- CCAGCCAACCTTTACACCAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Nsdhl (18194)
- Gene Description:
- NAD(P) dependent steroid dehydrogenase-like
- Transcript:
- RefSeq NM_010941.3 (CURRENT)
- Match location:
- Position 1142 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000041896)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCAGCCAACCTTTACACCAAT
- Hairpin Sequence:
-
5'-CCGG-CCAGCCAACCTTTACACCAAT-CTCGAG-ATTGGTGTAAAGGTTGGCTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAGCCAACCTTTACACCAATCTCGAGATTGGTGTAAAGGTTGGCTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAGCCAACCTTTACACCAATCTCGAGATTGGTGTAAAGGTTGGCTGG-3'
Other clones with same target sequence:
-
TRCN0000312097
