Construct: shRNA TRCN0000041896

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010941.3-1142s1c1
DNA Barcode:
CCAGCCAACCTTTACACCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nsdhl (18194)
Gene Description:
NAD(P) dependent steroid dehydrogenase-like
Transcript:
RefSeq NM_010941.3 (CURRENT)
Match location:
Position 1142 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041896)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCCAACCTTTACACCAAT
Hairpin Sequence:
5'-CCGG-CCAGCCAACCTTTACACCAAT-CTCGAG-ATTGGTGTAAAGGTTGGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCCAACCTTTACACCAATCTCGAGATTGGTGTAAAGGTTGGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCCAACCTTTACACCAATCTCGAGATTGGTGTAAAGGTTGGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000312097