Construct: shRNA TRCN0000041930

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011172.1-399s1c1
DNA Barcode:
GTTGGCTTTATCCTGGACTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prodh (19125)
Gene Description:
proline dehydrogenase
Transcript:
RefSeq NM_011172.1 (NON-CURRENT)
Match location:
Position 399 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041930)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGGCTTTATCCTGGACTAT
Hairpin Sequence:
5'-CCGG-GTTGGCTTTATCCTGGACTAT-CTCGAG-ATAGTCCAGGATAAAGCCAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGGCTTTATCCTGGACTATCTCGAGATAGTCCAGGATAAAGCCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGGCTTTATCCTGGACTATCTCGAGATAGTCCAGGATAAAGCCAAC-3'

Other clones with same target sequence:

(none)