Construct: shRNA TRCN0000041960

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_170778.1-192s1c1
DNA Barcode:
CGCAGCCAAGAAACTAGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dpyd (99586)
Gene Description:
dihydropyrimidine dehydrogenase
Transcript:
RefSeq NM_170778.1 (NON-CURRENT)
Match location:
Position 192 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041960)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGCCAAGAAACTAGACAA
Hairpin Sequence:
5'-CCGG-CGCAGCCAAGAAACTAGACAA-CTCGAG-TTGTCTAGTTTCTTGGCTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGCCAAGAAACTAGACAACTCGAGTTGTCTAGTTTCTTGGCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGCCAAGAAACTAGACAACTCGAGTTGTCTAGTTTCTTGGCTGCG-3'

Other clones with same target sequence:

(none)