Construct: shRNA TRCN0000041999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080436.1-624s1c1
DNA Barcode:
CATGGGCCGAATGTCTTTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rdh1 (107605)
Gene Description:
retinol dehydrogenase 1 (all trans)
Transcript:
RefSeq NM_080436.1 (NON-CURRENT)
Match location:
Position 624 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGGGCCGAATGTCTTTCTT
Hairpin Sequence:
5'-CCGG-CATGGGCCGAATGTCTTTCTT-CTCGAG-AAGAAAGACATTCGGCCCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGGGCCGAATGTCTTTCTTCTCGAGAAGAAAGACATTCGGCCCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGGGCCGAATGTCTTTCTTCTCGAGAAGAAAGACATTCGGCCCATG-3'

Other clones with same target sequence:

(none)