Construct: shRNA TRCN0000042005

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007409.2-336s1c1
DNA Barcode:
GATAAAGTCATTCCACTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adh1 (11522)
Gene Description:
alcohol dehydrogenase 1 (class I)
Transcript:
RefSeq NM_007409.2 (CURRENT)
Match location:
Position 336 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042005)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAAAGTCATTCCACTCTTT
Hairpin Sequence:
5'-CCGG-GATAAAGTCATTCCACTCTTT-CTCGAG-AAAGAGTGGAATGACTTTATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAAAGTCATTCCACTCTTTCTCGAGAAAGAGTGGAATGACTTTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAAAGTCATTCCACTCTTTCTCGAGAAAGAGTGGAATGACTTTATC-3'

Other clones with same target sequence:

(none)