Construct: shRNA TRCN0000042015

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027406.1-2622s1c1
DNA Barcode:
CGGGCCAATGCTACAGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aldh1l1 (107747)
Gene Description:
aldehyde dehydrogenase 1 family, member L1
Transcript:
RefSeq NM_027406.1 (CURRENT)
Match location:
Position 2622 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042015)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGCCAATGCTACAGAATTT
Hairpin Sequence:
5'-CCGG-CGGGCCAATGCTACAGAATTT-CTCGAG-AAATTCTGTAGCATTGGCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGCCAATGCTACAGAATTTCTCGAGAAATTCTGTAGCATTGGCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGCCAATGCTACAGAATTTCTCGAGAAATTCTGTAGCATTGGCCCG-3'

Other clones with same target sequence:

(none)