Construct: shRNA TRCN0000042022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009626.2-907s1c1
DNA Barcode:
GCAACACCATACAGTATACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adh7 (11529)
Gene Description:
alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide
Transcript:
RefSeq NM_009626.2 (NON-CURRENT)
Match location:
Position 907 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACACCATACAGTATACTT
Hairpin Sequence:
5'-CCGG-GCAACACCATACAGTATACTT-CTCGAG-AAGTATACTGTATGGTGTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACACCATACAGTATACTTCTCGAGAAGTATACTGTATGGTGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACACCATACAGTATACTTCTCGAGAAGTATACTGTATGGTGTTGC-3'

Other clones with same target sequence:

  1. TRCN0000308597