Construct: shRNA TRCN0000042023

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175094.2-1620s1c1
DNA Barcode:
GCGTATTTATTTAAGGCGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pdhx (27402)
Gene Description:
pyruvate dehydrogenase complex, component X
Transcript:
RefSeq NM_175094.2 (NON-CURRENT)
Match location:
Position 1620 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042023)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTATTTATTTAAGGCGAAA
Hairpin Sequence:
5'-CCGG-GCGTATTTATTTAAGGCGAAA-CTCGAG-TTTCGCCTTAAATAAATACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTATTTATTTAAGGCGAAACTCGAGTTTCGCCTTAAATAAATACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTATTTATTTAAGGCGAAACTCGAGTTTCGCCTTAAATAAATACGC-3'

Other clones with same target sequence:

(none)