Construct: shRNA TRCN0000042067

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009656.1-1081s1c1
DNA Barcode:
CGGACCTTCGTGCAGGAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aldh2 (11669)
Gene Description:
aldehyde dehydrogenase 2, mitochondrial
Transcript:
RefSeq NM_009656.1 (NON-CURRENT)
Match location:
Position 1081 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042067)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACCTTCGTGCAGGAGAAT
Hairpin Sequence:
5'-CCGG-CGGACCTTCGTGCAGGAGAAT-CTCGAG-ATTCTCCTGCACGAAGGTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACCTTCGTGCAGGAGAATCTCGAGATTCTCCTGCACGAAGGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACCTTCGTGCAGGAGAATCTCGAGATTCTCCTGCACGAAGGTCCG-3'

Other clones with same target sequence:

  1. TRCN0000308518