Construct: shRNA TRCN0000042074

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138600.1-1492s1c1
DNA Barcode:
CCCAATCCTCTATGTCTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aldh7a1 (110695)
Gene Description:
aldehyde dehydrogenase family 7, member A1
Transcript:
RefSeq NM_138600.1 (NON-CURRENT)
Match location:
Position 1492 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042074)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATCCTCTATGTCTTCAA
Hairpin Sequence:
5'-CCGG-CCCAATCCTCTATGTCTTCAA-CTCGAG-TTGAAGACATAGAGGATTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATCCTCTATGTCTTCAACTCGAGTTGAAGACATAGAGGATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATCCTCTATGTCTTCAACTCGAGTTGAAGACATAGAGGATTGGG-3'

Other clones with same target sequence:

  1. TRCN0000308582