Construct: shRNA TRCN0000042124

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133679.1-626s1c1
DNA Barcode:
CCCGTGTGATTGATGTGTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cryzl1 (66609)
Gene Description:
crystallin, zeta (quinone reductase)-like 1
Transcript:
RefSeq NM_133679.1 (NON-CURRENT)
Match location:
Position 626 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042124)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGTGTGATTGATGTGTCTA
Hairpin Sequence:
5'-CCGG-CCCGTGTGATTGATGTGTCTA-CTCGAG-TAGACACATCAATCACACGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTGTGATTGATGTGTCTACTCGAGTAGACACATCAATCACACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTGTGATTGATGTGTCTACTCGAGTAGACACATCAATCACACGGG-3'

Other clones with same target sequence:

  1. TRCN0000312384