Construct: shRNA TRCN0000042195

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010049.2-518s1c1
DNA Barcode:
GACAAGGATCATGCAGGAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dhfr (13361)
Gene Description:
dihydrofolate reductase
Transcript:
RefSeq NM_010049.2 (NON-CURRENT)
Match location:
Position 518 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042195)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAAGGATCATGCAGGAATT
Hairpin Sequence:
5'-CCGG-GACAAGGATCATGCAGGAATT-CTCGAG-AATTCCTGCATGATCCTTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAAGGATCATGCAGGAATTCTCGAGAATTCCTGCATGATCCTTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAAGGATCATGCAGGAATTCTCGAGAATTCCTGCATGATCCTTGTC-3'

Other clones with same target sequence:

  1. TRCN0000325291