Construct: shRNA TRCN0000042207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008012.1-188s1c1
DNA Barcode:
CGCCATATCGACTGCGCGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Akr1b8 (14187)
Gene Description:
aldo-keto reductase family 1, member B8
Transcript:
RefSeq NM_008012.1 (NON-CURRENT)
Match location:
Position 188 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCATATCGACTGCGCGTAT
Hairpin Sequence:
5'-CCGG-CGCCATATCGACTGCGCGTAT-CTCGAG-ATACGCGCAGTCGATATGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCATATCGACTGCGCGTATCTCGAGATACGCGCAGTCGATATGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCATATCGACTGCGCGTATCTCGAGATACGCGCAGTCGATATGGCG-3'

Other clones with same target sequence:

(none)