Construct: shRNA TRCN0000042236

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018859.1-855s1c1
DNA Barcode:
CCTAGACAAGAACCTCCGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Akr1e1 (56043)
Gene Description:
aldo-keto reductase family 1, member E1
Transcript:
RefSeq NM_018859.1 (NON-CURRENT)
Match location:
Position 855 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042236)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGACAAGAACCTCCGTTT
Hairpin Sequence:
5'-CCGG-CCTAGACAAGAACCTCCGTTT-CTCGAG-AAACGGAGGTTCTTGTCTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGACAAGAACCTCCGTTTCTCGAGAAACGGAGGTTCTTGTCTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGACAAGAACCTCCGTTTCTCGAGAAACGGAGGTTCTTGTCTAGG-3'

Other clones with same target sequence:

(none)