Construct: shRNA TRCN0000042415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008675.1-173s1c1
DNA Barcode:
GCCAAGAACATCACGCAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nbl1 (17965)
Gene Description:
neuroblastoma, suppression of tumorigenicity 1
Transcript:
RefSeq NM_008675.1 (NON-CURRENT)
Match location:
Position 173 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGAACATCACGCAGATT
Hairpin Sequence:
5'-CCGG-GCCAAGAACATCACGCAGATT-CTCGAG-AATCTGCGTGATGTTCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGAACATCACGCAGATTCTCGAGAATCTGCGTGATGTTCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGAACATCACGCAGATTCTCGAGAATCTGCGTGATGTTCTTGGC-3'

Other clones with same target sequence:

(none)