Construct: shRNA TRCN0000042453

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178902.3-3210s1c1
DNA Barcode:
GCCTGTATTAACCAAGTCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtus1 (102103)
Gene Description:
mitochondrial tumor suppressor 1
Transcript:
RefSeq NM_178902.3 (NON-CURRENT)
Match location:
Position 3210 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042453)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGTATTAACCAAGTCATA
Hairpin Sequence:
5'-CCGG-GCCTGTATTAACCAAGTCATA-CTCGAG-TATGACTTGGTTAATACAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGTATTAACCAAGTCATACTCGAGTATGACTTGGTTAATACAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGTATTAACCAAGTCATACTCGAGTATGACTTGGTTAATACAGGC-3'

Other clones with same target sequence:

(none)