Construct: shRNA TRCN0000042531
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_011057.2-1006s1c1
- DNA Barcode:
- GCCTGCAAGTGTGAGACAGTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Pdgfb (18591)
- Gene Description:
- platelet derived growth factor, B polypeptide
- Transcript:
- RefSeq NM_011057.2 (NON-CURRENT)
- Match location:
- Position 1006 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000042531)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCTGCAAGTGTGAGACAGTA
- Hairpin Sequence:
-
5'-CCGG-GCCTGCAAGTGTGAGACAGTA-CTCGAG-TACTGTCTCACACTTGCAGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTGCAAGTGTGAGACAGTACTCGAGTACTGTCTCACACTTGCAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTGCAAGTGTGAGACAGTACTCGAGTACTGTCTCACACTTGCAGGC-3'
Other clones with same target sequence:
-
TRCN0000327325
