Construct: shRNA TRCN0000042559
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_009764.2-2397s1c1
- DNA Barcode:
- GCTCAGTGTATGACTCAGTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Brca1 (12189)
- Gene Description:
- breast cancer 1, early onset
- Transcript:
- RefSeq NM_009764.2 (NON-CURRENT)
- Match location:
- Position 2397 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000042559)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTCAGTGTATGACTCAGTTT
- Hairpin Sequence:
-
5'-CCGG-GCTCAGTGTATGACTCAGTTT-CTCGAG-AAACTGAGTCATACACTGAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTCAGTGTATGACTCAGTTTCTCGAGAAACTGAGTCATACACTGAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTCAGTGTATGACTCAGTTTCTCGAGAAACTGAGTCATACACTGAGC-3'
Other clones with same target sequence:
-
TRCN0000326125
