Construct: shRNA TRCN0000042624

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023653.3-763s1c1
DNA Barcode:
CGACTATCTCTGGAGGAAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wnt2 (22413)
Gene Description:
wingless-type MMTV integration site family, member 2
Transcript:
RefSeq NM_023653.3 (NON-CURRENT)
Match location:
Position 763 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042624)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACTATCTCTGGAGGAAGTA
Hairpin Sequence:
5'-CCGG-CGACTATCTCTGGAGGAAGTA-CTCGAG-TACTTCCTCCAGAGATAGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACTATCTCTGGAGGAAGTACTCGAGTACTTCCTCCAGAGATAGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACTATCTCTGGAGGAAGTACTCGAGTACTTCCTCCAGAGATAGTCG-3'

Other clones with same target sequence:

(none)