Construct: shRNA TRCN0000042688

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008037.3-995s1c1
DNA Barcode:
CGGCACTTCAAACCTTGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fosl2 (14284)
Gene Description:
fos-like antigen 2
Transcript:
RefSeq NM_008037.3 (NON-CURRENT)
Match location:
Position 995 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042688)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCACTTCAAACCTTGTCTT
Hairpin Sequence:
5'-CCGG-CGGCACTTCAAACCTTGTCTT-CTCGAG-AAGACAAGGTTTGAAGTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCACTTCAAACCTTGTCTTCTCGAGAAGACAAGGTTTGAAGTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCACTTCAAACCTTGTCTTCTCGAGAAGACAAGGTTTGAAGTGCCG-3'

Other clones with same target sequence:

(none)