Construct: shRNA TRCN0000042724

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011647.1-1352s1c1
DNA Barcode:
CCTCTCTCTTAAACTTGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tsc2 (22084)
Gene Description:
tuberous sclerosis 2
Transcript:
RefSeq NM_011647.1 (NON-CURRENT)
Match location:
Position 1352 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042724)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCTCTTAAACTTGATAT
Hairpin Sequence:
5'-CCGG-CCTCTCTCTTAAACTTGATAT-CTCGAG-ATATCAAGTTTAAGAGAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCTCTTAAACTTGATATCTCGAGATATCAAGTTTAAGAGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCTCTTAAACTTGATATCTCGAGATATCAAGTTTAAGAGAGAGG-3'

Other clones with same target sequence:

  1. TRCN0000326277