Construct: shRNA TRCN0000042727

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011647.1-1843s1c1
DNA Barcode:
CGAGTGTATGAGAGCCTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tsc2 (22084)
Gene Description:
tuberous sclerosis 2
Transcript:
RefSeq NM_011647.1 (NON-CURRENT)
Match location:
Position 1843 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042727)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGTGTATGAGAGCCTCATT
Hairpin Sequence:
5'-CCGG-CGAGTGTATGAGAGCCTCATT-CTCGAG-AATGAGGCTCTCATACACTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGTGTATGAGAGCCTCATTCTCGAGAATGAGGCTCTCATACACTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGTGTATGAGAGCCTCATTCTCGAGAATGAGGCTCTCATACACTCG-3'

Other clones with same target sequence:

  1. TRCN0000326275