Construct: shRNA TRCN0000042747

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009487.1-1539s1c1
DNA Barcode:
CCAAAGAGTCAACAACTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn2r89 (22301)
Gene Description:
vomeronasal 2, receptor 89
Transcript:
RefSeq NM_009487.1 (CURRENT)
Match location:
Position 1539 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042747)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGAGTCAACAACTTCAT
Hairpin Sequence:
5'-CCGG-CCAAAGAGTCAACAACTTCAT-CTCGAG-ATGAAGTTGTTGACTCTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGAGTCAACAACTTCATCTCGAGATGAAGTTGTTGACTCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGAGTCAACAACTTCATCTCGAGATGAAGTTGTTGACTCTTTGG-3'

Other clones with same target sequence:

(none)