Construct: shRNA TRCN0000042777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019444.1-321s1c1
DNA Barcode:
GACTTTGATTAGCAGGCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ramp2 (54409)
Gene Description:
receptor (calcitonin) activity modifying protein 2
Transcript:
RefSeq NM_019444.1 (NON-CURRENT)
Match location:
Position 321 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTTTGATTAGCAGGCATTA
Hairpin Sequence:
5'-CCGG-GACTTTGATTAGCAGGCATTA-CTCGAG-TAATGCCTGCTAATCAAAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTTTGATTAGCAGGCATTACTCGAGTAATGCCTGCTAATCAAAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTTTGATTAGCAGGCATTACTCGAGTAATGCCTGCTAATCAAAGTC-3'

Other clones with same target sequence:

(none)