Construct: shRNA TRCN0000042876

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003042.2-1300s1c1
DNA Barcode:
GAGCTACAACTCTTTCCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC6A1 (6529)
Gene Description:
solute carrier family 6 member 1
Transcript:
RefSeq NM_003042.2 (NON-CURRENT)
Match location:
Position 1300 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042876)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCTACAACTCTTTCCACAA
Hairpin Sequence:
5'-CCGG-GAGCTACAACTCTTTCCACAA-CTCGAG-TTGTGGAAAGAGTTGTAGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCTACAACTCTTTCCACAACTCGAGTTGTGGAAAGAGTTGTAGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCTACAACTCTTTCCACAACTCGAGTTGTGGAAAGAGTTGTAGCTC-3'

Other clones with same target sequence:

(none)